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phospho perk  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phospho perk
    Phospho Perk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 935 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+perk+thr980/Phospho-PERK+(Thr980)+Rabbit+mAb/pmc12407559-89-101-104
    Average 96 stars, based on 935 article reviews
    phospho perk - by Bioz Stars, 2026-10
    96/100 stars

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    Article Title: Glucose-driven histone lactylation promotes the immunosuppressive activity of monocyte-derived macrophages in glioblastoma.
    Article Snippet: In brief There are no effective approaches to overcome the immunosuppressive activity of tumor-associated macrophages (TAMs).. De Leo et al. report that monocyte-derived macrophages (MDMs), but not microglia (MGs), are major contributors to the immunosuppression associated with TAMs in glioblastoma and demonstrate that PERK-perturbed glucose metabolism drives MDM immunosuppressive programs via histone lactylation.. Targeting of PERKdriven histone lactylation boosts the effect of immunotherapy.

    Article Title: Acute Exercise Increases GDF15 and Unfolded Protein Response/Integrated Stress Response in Muscle in Type 2 Diabetes.
    Article Snippet: Context: Regular exercise is a key prevention strategy for obesity and type 2 diabetes (T2D).. Exerkines secreted in response to exercise or recovery may contribute to improved systemic metabolism.. Conversely, an impaired exerkine response to exercise and recovery may contribute to cardiometabolic diseases.

    Western Blot:

    Article Title: From Ca 2 + dysregulation to heart failure: β-adrenoceptor activation by RKIP postpones molecular damages and subsequent cardiac dysfunction in mice carrying mutant PLN R9C by correction of aberrant Ca 2+ -handling.
    Article Snippet: .. We used specific antibodies to the following proteins for immunoblotting: pPLN (S16) (A010–12, Badrilla; 1:2000), antibody 2081 was generated against a synthetic peptide containing amino acids 9–28 of mouse PLNR9C (CSAIRRASTIEMPQQARQNL) rabbit antisera were used at a concentration of 20 ng/ml, Calreticulin (sc-11398, Santa Cruz, 1:2000), eIF2α (#9722, 1:1000, Cell Signaling), Gβ (sc-378, Santa Cruz; 1:5000), GRK-2 (sc-562, Santa Cruz; 1:2000), GRP-78 (sc-166490, Santa Cruz, 1:2000), HAX-1 (610825, BD Bioscience, 1:2.000), NDUFV-1 (sc133808, Santa Cruz; 1:1000), PERK (#3192, 1:1000, Cell Signaling), phospho-eIF2α (Ser51) (#9721, 1:1000, Cell Signaling), phospho-PERK (Thr980) (#3179, 1:1000, Cell Signaling), phosphor-RKIP (Ser153) (sc135779, 1:2000, Santa Cruz), phospho-(Ser/Thr) PKA Substrate (#9621, Cell Signaling), PKAα cat (sc-28315, Santa Cruz, 1:2000), PLN (sc-393990, Santa Cruz, for IP-experiments: 10 μl per sample), PLN (A010–14, Badrilla, 1:5000), (Porin (ab15895, abcam, 1:5000), RKIP (FL-187) (sc-28837, Santa Cruz; 1:1000), SERCA (sc-8095, Santa Cruz; 1:10000), Total OXPHOS Rodent WB Antibody cocktail (ab110413, abcam, 1:2500), ubiquitin (P4D1) (sc-8017, Santa Cruz, 1:1000), peroxidase conjugated goat anti-rabbit (Dianova, Jackson ImmunoResearch, 111–035–144; 1:10000), peroxidase conjugated goat antimouse (Dianova, Jackson ImmunoResearch, 115–035–003; 1:10000), peroxidase conjugated donkey anti-goat (sc-2020, Santa Cruz, 1:5000). ..

    Article Title: Fig. S3 (a) identification by qPCR of the screen genes and small GTPase mRNA expression normalized by 18 s; (b) identification of EIF2AK3, eIF2α, and eIF2β mRNA expression by qPCR in TCMK-1 treated with PERKi or ISRIB after CDDP; (c and d) The PERK activation and mRNA expression in smgGDS knockdown cell line rescue assay. qPCR, quantitative polymerase chain reaction; CDDP, cisplatin; PERKi, selective PERK phosphorylation inhibitor;
    Article Snippet: .. 4 Fig. S4 Flow cytometry gating strategy 5 Table S1 Antibodies Antibody Source Catalog Dilution (WB) Dilution (IF and IC) RAP1GDS1 mouse mAb Santa Cruz Biotechnology, Dallas, TX, USA sc-390003 1:200 1:25 Cadherin 16 Rabbit pAb ABclonal, Wuhan, China A7882 - 1:200 NGAL Rabbit pAb ABclonal, Wuhan, China A2092 - 1:200 KIM-1 Rabbit mAb Cell Signaling Technology [CST], Danvers, MA #14971 1:1000 - NGAL Rabbit mAb CST, Danvers, MA #44058 1:1000 - PERK (D11A8) Rabbit mAb CST, Danvers, MA #5683 1:1000 - Phospho-PERK (Thr980) (16F8) Rabbit mAb CST, Danvers, MA #3179 1:1000 - Phospho-eIF2α-S51 Rabbit mAb ABclonal, Wuhan, China AP0692 1:1000 - eIF2α Rabbit mAb ABclonal, Wuhan, China A21221 1:1000 - GRP78 mouse mAb Santa Cruz Biotechnology, Dallas, TX, USA sc-166490 1:1000 - ATF-4 (D4B8) Rabbit mAb CST, Danvers, MA #11815 1:1000 - DDIT3/CHOP Rabbit mAb ABclonal, Wuhan, China A20987 1:1000 - Active + pro Caspase-3 Rabbit mAb ABclonal, Wuhan, China A19654 1:1000 - 6 Antibody Source Catalog Dilution (WB) Dilution (IF and IC) PARP (46D11) Rabbit mAb CST, Danvers, MA #9532 1:1000 - Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb CST, Danvers, MA #9625 1:1000 - Bax (E4U1V) Rabbit mAb CST, Danvers, MA #41162 1:1000 - Bcl-2 Rabbit mAb ABclonal, Wuhan, China A19693 1:1000 - β-actin Rabbit mAb ABclonal, Wuhan, China AC026 1:200000 DyLight 594 Conjugated AffiniPure Goat Antimouse IgG (H+L) BOSTER, Beijing, China BA1141 - 1:200 FITC Conjugated AffiniPure Goat Antirabbit IgG (H+L) BOSTER, Beijing, China BM2012 - 1:100 WB, Western blot; IF, immunofluorescence; IC, immunochemistry. ..

    Article Title: p21-activated kinase 4 suppresses fatty acid β-oxidation and ketogenesis by phosphorylating NCoR1
    Article Snippet: .. Antibodies used for Western blotting, immunofluorescence and immunohistochemical analyses Antibody Catalogue No. Dilution Fold Company Western Blot PAK4 (G222) 62690 1:2500 Cell Signaling Technology p-PAK4 (S474) 3241 1:2500 Cell Signaling Technology HSP90 (C45G5) 4877 1:2500 Cell Signaling Technology Ubiquitin (E4I2J) 43124 1:2500 Cell Signaling Technology HMGCS2 (D3U1A) 20940 1:2500 Cell Signaling Technology Phospho-PKA Substrate (100G7E) 9624 1:2500 Cell Signaling Technology Phospho-(Ser/Thr) 9631 1:2500 Cell Signaling Technology Sirt1 2310 1:2500 Cell Signaling Technology Sirt6 (D8D12) 12486 1:2500 Cell Signaling Technology Phospho-Akt (Ser473) (D9E) 4060 1:2500 Cell Signaling Technology Akt 9272 1:2500 Cell Signaling Technology Acetylated-Lysine 9441 1:2500 Cell Signaling Technology CHOP (L63F7) 2895 1:2500 Cell Signaling Technology Phospho-S6 Ribosomal Protein (Ser240/244) 2215 1:2500 Cell Signaling Technology S6 Ribosomal Protein (5G10) 2217 1:2500 Cell Signaling Technology NCoR1 5948 1:2500 Cell Signaling Technology NEDD4 2740 1:2500 Cell Signaling Technology ATF-6 (D4Z8V) 65880 1:2500 Cell Signaling Technology Phospho-PERK (Thr980) (16F8) 3179 1:2500 Cell Signaling Technology PAK4 (B-3) SC-390507 1:2500 Santa Cruz Biotechnology FGF21 (H-105) SC-292879 1:2500 Santa Cruz Biotechnology PPARα (H-2) SC-398394 1:2500 Santa Cruz Biotechnology Sirt4 3224 1:2500 Bioworld Technology LaminB1 (L75) BS3547 1:2500 Bioworld Technology GAPDH (A531) AP0066 1:2500 Bioworld Technology Sirt5 (aa30-46) LS-B2060 1:2500 LifeSpan Biosciences Sirt7 ( aa317-366) LS-B1566 1:2500 LifeSpan Biosciences FoxO1 (Acetyl-Lys294) LS-C800723 1:2500 LifeSpan Biosciences Sirt2 (EPR20411-105) ab211033 1:2500 Abcam Sirt3 ab189860 1:2500 Abcam GRP78 BiP ab21685 1:2500 Abcam NCOR2/SMRT ab24551 1:2500 Abcam CPT1A (8F6AE9) ab128568 1:2500 Abcam T-OXPHOS ab110413 1:2500 Abcam THRβ ab53170 1:2500 Abcam p-IRE1α ab48187 1:2500 Abcam MDM2 (2A10) ab16895 1:2500 Abcam LXRα 14351-1-AP 1:2500 Proteintech Proximity Ligation Assay NCoR1 5948 1:100 Cell Signaling Technology p300 (D8Z4E) 86377 1:100 Cell Signaling Technology PPARα (H-2) SC-398394 1:100 Santa Cruz Biotechnology Immunofluorescence NCoR1 5948 1:100 Cell Signaling Technology PPARα (H-2) SC-398394 1:100 Santa Cruz Biotechnology Supplementary Table 4. ..

    Generated:

    Article Title: From Ca 2 + dysregulation to heart failure: β-adrenoceptor activation by RKIP postpones molecular damages and subsequent cardiac dysfunction in mice carrying mutant PLN R9C by correction of aberrant Ca 2+ -handling.
    Article Snippet: .. We used specific antibodies to the following proteins for immunoblotting: pPLN (S16) (A010–12, Badrilla; 1:2000), antibody 2081 was generated against a synthetic peptide containing amino acids 9–28 of mouse PLNR9C (CSAIRRASTIEMPQQARQNL) rabbit antisera were used at a concentration of 20 ng/ml, Calreticulin (sc-11398, Santa Cruz, 1:2000), eIF2α (#9722, 1:1000, Cell Signaling), Gβ (sc-378, Santa Cruz; 1:5000), GRK-2 (sc-562, Santa Cruz; 1:2000), GRP-78 (sc-166490, Santa Cruz, 1:2000), HAX-1 (610825, BD Bioscience, 1:2.000), NDUFV-1 (sc133808, Santa Cruz; 1:1000), PERK (#3192, 1:1000, Cell Signaling), phospho-eIF2α (Ser51) (#9721, 1:1000, Cell Signaling), phospho-PERK (Thr980) (#3179, 1:1000, Cell Signaling), phosphor-RKIP (Ser153) (sc135779, 1:2000, Santa Cruz), phospho-(Ser/Thr) PKA Substrate (#9621, Cell Signaling), PKAα cat (sc-28315, Santa Cruz, 1:2000), PLN (sc-393990, Santa Cruz, for IP-experiments: 10 μl per sample), PLN (A010–14, Badrilla, 1:5000), (Porin (ab15895, abcam, 1:5000), RKIP (FL-187) (sc-28837, Santa Cruz; 1:1000), SERCA (sc-8095, Santa Cruz; 1:10000), Total OXPHOS Rodent WB Antibody cocktail (ab110413, abcam, 1:2500), ubiquitin (P4D1) (sc-8017, Santa Cruz, 1:1000), peroxidase conjugated goat anti-rabbit (Dianova, Jackson ImmunoResearch, 111–035–144; 1:10000), peroxidase conjugated goat antimouse (Dianova, Jackson ImmunoResearch, 115–035–003; 1:10000), peroxidase conjugated donkey anti-goat (sc-2020, Santa Cruz, 1:5000). ..

    Concentration Assay:

    Article Title: From Ca 2 + dysregulation to heart failure: β-adrenoceptor activation by RKIP postpones molecular damages and subsequent cardiac dysfunction in mice carrying mutant PLN R9C by correction of aberrant Ca 2+ -handling.
    Article Snippet: .. We used specific antibodies to the following proteins for immunoblotting: pPLN (S16) (A010–12, Badrilla; 1:2000), antibody 2081 was generated against a synthetic peptide containing amino acids 9–28 of mouse PLNR9C (CSAIRRASTIEMPQQARQNL) rabbit antisera were used at a concentration of 20 ng/ml, Calreticulin (sc-11398, Santa Cruz, 1:2000), eIF2α (#9722, 1:1000, Cell Signaling), Gβ (sc-378, Santa Cruz; 1:5000), GRK-2 (sc-562, Santa Cruz; 1:2000), GRP-78 (sc-166490, Santa Cruz, 1:2000), HAX-1 (610825, BD Bioscience, 1:2.000), NDUFV-1 (sc133808, Santa Cruz; 1:1000), PERK (#3192, 1:1000, Cell Signaling), phospho-eIF2α (Ser51) (#9721, 1:1000, Cell Signaling), phospho-PERK (Thr980) (#3179, 1:1000, Cell Signaling), phosphor-RKIP (Ser153) (sc135779, 1:2000, Santa Cruz), phospho-(Ser/Thr) PKA Substrate (#9621, Cell Signaling), PKAα cat (sc-28315, Santa Cruz, 1:2000), PLN (sc-393990, Santa Cruz, for IP-experiments: 10 μl per sample), PLN (A010–14, Badrilla, 1:5000), (Porin (ab15895, abcam, 1:5000), RKIP (FL-187) (sc-28837, Santa Cruz; 1:1000), SERCA (sc-8095, Santa Cruz; 1:10000), Total OXPHOS Rodent WB Antibody cocktail (ab110413, abcam, 1:2500), ubiquitin (P4D1) (sc-8017, Santa Cruz, 1:1000), peroxidase conjugated goat anti-rabbit (Dianova, Jackson ImmunoResearch, 111–035–144; 1:10000), peroxidase conjugated goat antimouse (Dianova, Jackson ImmunoResearch, 115–035–003; 1:10000), peroxidase conjugated donkey anti-goat (sc-2020, Santa Cruz, 1:5000). ..

    Ubiquitin Proteomics:

    Article Title: From Ca 2 + dysregulation to heart failure: β-adrenoceptor activation by RKIP postpones molecular damages and subsequent cardiac dysfunction in mice carrying mutant PLN R9C by correction of aberrant Ca 2+ -handling.
    Article Snippet: .. We used specific antibodies to the following proteins for immunoblotting: pPLN (S16) (A010–12, Badrilla; 1:2000), antibody 2081 was generated against a synthetic peptide containing amino acids 9–28 of mouse PLNR9C (CSAIRRASTIEMPQQARQNL) rabbit antisera were used at a concentration of 20 ng/ml, Calreticulin (sc-11398, Santa Cruz, 1:2000), eIF2α (#9722, 1:1000, Cell Signaling), Gβ (sc-378, Santa Cruz; 1:5000), GRK-2 (sc-562, Santa Cruz; 1:2000), GRP-78 (sc-166490, Santa Cruz, 1:2000), HAX-1 (610825, BD Bioscience, 1:2.000), NDUFV-1 (sc133808, Santa Cruz; 1:1000), PERK (#3192, 1:1000, Cell Signaling), phospho-eIF2α (Ser51) (#9721, 1:1000, Cell Signaling), phospho-PERK (Thr980) (#3179, 1:1000, Cell Signaling), phosphor-RKIP (Ser153) (sc135779, 1:2000, Santa Cruz), phospho-(Ser/Thr) PKA Substrate (#9621, Cell Signaling), PKAα cat (sc-28315, Santa Cruz, 1:2000), PLN (sc-393990, Santa Cruz, for IP-experiments: 10 μl per sample), PLN (A010–14, Badrilla, 1:5000), (Porin (ab15895, abcam, 1:5000), RKIP (FL-187) (sc-28837, Santa Cruz; 1:1000), SERCA (sc-8095, Santa Cruz; 1:10000), Total OXPHOS Rodent WB Antibody cocktail (ab110413, abcam, 1:2500), ubiquitin (P4D1) (sc-8017, Santa Cruz, 1:1000), peroxidase conjugated goat anti-rabbit (Dianova, Jackson ImmunoResearch, 111–035–144; 1:10000), peroxidase conjugated goat antimouse (Dianova, Jackson ImmunoResearch, 115–035–003; 1:10000), peroxidase conjugated donkey anti-goat (sc-2020, Santa Cruz, 1:5000). ..

    Article Title: p21-activated kinase 4 suppresses fatty acid β-oxidation and ketogenesis by phosphorylating NCoR1
    Article Snippet: .. Antibodies used for Western blotting, immunofluorescence and immunohistochemical analyses Antibody Catalogue No. Dilution Fold Company Western Blot PAK4 (G222) 62690 1:2500 Cell Signaling Technology p-PAK4 (S474) 3241 1:2500 Cell Signaling Technology HSP90 (C45G5) 4877 1:2500 Cell Signaling Technology Ubiquitin (E4I2J) 43124 1:2500 Cell Signaling Technology HMGCS2 (D3U1A) 20940 1:2500 Cell Signaling Technology Phospho-PKA Substrate (100G7E) 9624 1:2500 Cell Signaling Technology Phospho-(Ser/Thr) 9631 1:2500 Cell Signaling Technology Sirt1 2310 1:2500 Cell Signaling Technology Sirt6 (D8D12) 12486 1:2500 Cell Signaling Technology Phospho-Akt (Ser473) (D9E) 4060 1:2500 Cell Signaling Technology Akt 9272 1:2500 Cell Signaling Technology Acetylated-Lysine 9441 1:2500 Cell Signaling Technology CHOP (L63F7) 2895 1:2500 Cell Signaling Technology Phospho-S6 Ribosomal Protein (Ser240/244) 2215 1:2500 Cell Signaling Technology S6 Ribosomal Protein (5G10) 2217 1:2500 Cell Signaling Technology NCoR1 5948 1:2500 Cell Signaling Technology NEDD4 2740 1:2500 Cell Signaling Technology ATF-6 (D4Z8V) 65880 1:2500 Cell Signaling Technology Phospho-PERK (Thr980) (16F8) 3179 1:2500 Cell Signaling Technology PAK4 (B-3) SC-390507 1:2500 Santa Cruz Biotechnology FGF21 (H-105) SC-292879 1:2500 Santa Cruz Biotechnology PPARα (H-2) SC-398394 1:2500 Santa Cruz Biotechnology Sirt4 3224 1:2500 Bioworld Technology LaminB1 (L75) BS3547 1:2500 Bioworld Technology GAPDH (A531) AP0066 1:2500 Bioworld Technology Sirt5 (aa30-46) LS-B2060 1:2500 LifeSpan Biosciences Sirt7 ( aa317-366) LS-B1566 1:2500 LifeSpan Biosciences FoxO1 (Acetyl-Lys294) LS-C800723 1:2500 LifeSpan Biosciences Sirt2 (EPR20411-105) ab211033 1:2500 Abcam Sirt3 ab189860 1:2500 Abcam GRP78 BiP ab21685 1:2500 Abcam NCOR2/SMRT ab24551 1:2500 Abcam CPT1A (8F6AE9) ab128568 1:2500 Abcam T-OXPHOS ab110413 1:2500 Abcam THRβ ab53170 1:2500 Abcam p-IRE1α ab48187 1:2500 Abcam MDM2 (2A10) ab16895 1:2500 Abcam LXRα 14351-1-AP 1:2500 Proteintech Proximity Ligation Assay NCoR1 5948 1:100 Cell Signaling Technology p300 (D8Z4E) 86377 1:100 Cell Signaling Technology PPARα (H-2) SC-398394 1:100 Santa Cruz Biotechnology Immunofluorescence NCoR1 5948 1:100 Cell Signaling Technology PPARα (H-2) SC-398394 1:100 Santa Cruz Biotechnology Supplementary Table 4. ..

    Flow Cytometry:

    Article Title: Fig. S3 (a) identification by qPCR of the screen genes and small GTPase mRNA expression normalized by 18 s; (b) identification of EIF2AK3, eIF2α, and eIF2β mRNA expression by qPCR in TCMK-1 treated with PERKi or ISRIB after CDDP; (c and d) The PERK activation and mRNA expression in smgGDS knockdown cell line rescue assay. qPCR, quantitative polymerase chain reaction; CDDP, cisplatin; PERKi, selective PERK phosphorylation inhibitor;
    Article Snippet: .. 4 Fig. S4 Flow cytometry gating strategy 5 Table S1 Antibodies Antibody Source Catalog Dilution (WB) Dilution (IF and IC) RAP1GDS1 mouse mAb Santa Cruz Biotechnology, Dallas, TX, USA sc-390003 1:200 1:25 Cadherin 16 Rabbit pAb ABclonal, Wuhan, China A7882 - 1:200 NGAL Rabbit pAb ABclonal, Wuhan, China A2092 - 1:200 KIM-1 Rabbit mAb Cell Signaling Technology [CST], Danvers, MA #14971 1:1000 - NGAL Rabbit mAb CST, Danvers, MA #44058 1:1000 - PERK (D11A8) Rabbit mAb CST, Danvers, MA #5683 1:1000 - Phospho-PERK (Thr980) (16F8) Rabbit mAb CST, Danvers, MA #3179 1:1000 - Phospho-eIF2α-S51 Rabbit mAb ABclonal, Wuhan, China AP0692 1:1000 - eIF2α Rabbit mAb ABclonal, Wuhan, China A21221 1:1000 - GRP78 mouse mAb Santa Cruz Biotechnology, Dallas, TX, USA sc-166490 1:1000 - ATF-4 (D4B8) Rabbit mAb CST, Danvers, MA #11815 1:1000 - DDIT3/CHOP Rabbit mAb ABclonal, Wuhan, China A20987 1:1000 - Active + pro Caspase-3 Rabbit mAb ABclonal, Wuhan, China A19654 1:1000 - 6 Antibody Source Catalog Dilution (WB) Dilution (IF and IC) PARP (46D11) Rabbit mAb CST, Danvers, MA #9532 1:1000 - Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb CST, Danvers, MA #9625 1:1000 - Bax (E4U1V) Rabbit mAb CST, Danvers, MA #41162 1:1000 - Bcl-2 Rabbit mAb ABclonal, Wuhan, China A19693 1:1000 - β-actin Rabbit mAb ABclonal, Wuhan, China AC026 1:200000 DyLight 594 Conjugated AffiniPure Goat Antimouse IgG (H+L) BOSTER, Beijing, China BA1141 - 1:200 FITC Conjugated AffiniPure Goat Antirabbit IgG (H+L) BOSTER, Beijing, China BM2012 - 1:100 WB, Western blot; IF, immunofluorescence; IC, immunochemistry. ..

    Immunofluorescence:

    Article Title: Fig. S3 (a) identification by qPCR of the screen genes and small GTPase mRNA expression normalized by 18 s; (b) identification of EIF2AK3, eIF2α, and eIF2β mRNA expression by qPCR in TCMK-1 treated with PERKi or ISRIB after CDDP; (c and d) The PERK activation and mRNA expression in smgGDS knockdown cell line rescue assay. qPCR, quantitative polymerase chain reaction; CDDP, cisplatin; PERKi, selective PERK phosphorylation inhibitor;
    Article Snippet: .. 4 Fig. S4 Flow cytometry gating strategy 5 Table S1 Antibodies Antibody Source Catalog Dilution (WB) Dilution (IF and IC) RAP1GDS1 mouse mAb Santa Cruz Biotechnology, Dallas, TX, USA sc-390003 1:200 1:25 Cadherin 16 Rabbit pAb ABclonal, Wuhan, China A7882 - 1:200 NGAL Rabbit pAb ABclonal, Wuhan, China A2092 - 1:200 KIM-1 Rabbit mAb Cell Signaling Technology [CST], Danvers, MA #14971 1:1000 - NGAL Rabbit mAb CST, Danvers, MA #44058 1:1000 - PERK (D11A8) Rabbit mAb CST, Danvers, MA #5683 1:1000 - Phospho-PERK (Thr980) (16F8) Rabbit mAb CST, Danvers, MA #3179 1:1000 - Phospho-eIF2α-S51 Rabbit mAb ABclonal, Wuhan, China AP0692 1:1000 - eIF2α Rabbit mAb ABclonal, Wuhan, China A21221 1:1000 - GRP78 mouse mAb Santa Cruz Biotechnology, Dallas, TX, USA sc-166490 1:1000 - ATF-4 (D4B8) Rabbit mAb CST, Danvers, MA #11815 1:1000 - DDIT3/CHOP Rabbit mAb ABclonal, Wuhan, China A20987 1:1000 - Active + pro Caspase-3 Rabbit mAb ABclonal, Wuhan, China A19654 1:1000 - 6 Antibody Source Catalog Dilution (WB) Dilution (IF and IC) PARP (46D11) Rabbit mAb CST, Danvers, MA #9532 1:1000 - Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb CST, Danvers, MA #9625 1:1000 - Bax (E4U1V) Rabbit mAb CST, Danvers, MA #41162 1:1000 - Bcl-2 Rabbit mAb ABclonal, Wuhan, China A19693 1:1000 - β-actin Rabbit mAb ABclonal, Wuhan, China AC026 1:200000 DyLight 594 Conjugated AffiniPure Goat Antimouse IgG (H+L) BOSTER, Beijing, China BA1141 - 1:200 FITC Conjugated AffiniPure Goat Antirabbit IgG (H+L) BOSTER, Beijing, China BM2012 - 1:100 WB, Western blot; IF, immunofluorescence; IC, immunochemistry. ..

    Article Title: p21-activated kinase 4 suppresses fatty acid β-oxidation and ketogenesis by phosphorylating NCoR1
    Article Snippet: .. Antibodies used for Western blotting, immunofluorescence and immunohistochemical analyses Antibody Catalogue No. Dilution Fold Company Western Blot PAK4 (G222) 62690 1:2500 Cell Signaling Technology p-PAK4 (S474) 3241 1:2500 Cell Signaling Technology HSP90 (C45G5) 4877 1:2500 Cell Signaling Technology Ubiquitin (E4I2J) 43124 1:2500 Cell Signaling Technology HMGCS2 (D3U1A) 20940 1:2500 Cell Signaling Technology Phospho-PKA Substrate (100G7E) 9624 1:2500 Cell Signaling Technology Phospho-(Ser/Thr) 9631 1:2500 Cell Signaling Technology Sirt1 2310 1:2500 Cell Signaling Technology Sirt6 (D8D12) 12486 1:2500 Cell Signaling Technology Phospho-Akt (Ser473) (D9E) 4060 1:2500 Cell Signaling Technology Akt 9272 1:2500 Cell Signaling Technology Acetylated-Lysine 9441 1:2500 Cell Signaling Technology CHOP (L63F7) 2895 1:2500 Cell Signaling Technology Phospho-S6 Ribosomal Protein (Ser240/244) 2215 1:2500 Cell Signaling Technology S6 Ribosomal Protein (5G10) 2217 1:2500 Cell Signaling Technology NCoR1 5948 1:2500 Cell Signaling Technology NEDD4 2740 1:2500 Cell Signaling Technology ATF-6 (D4Z8V) 65880 1:2500 Cell Signaling Technology Phospho-PERK (Thr980) (16F8) 3179 1:2500 Cell Signaling Technology PAK4 (B-3) SC-390507 1:2500 Santa Cruz Biotechnology FGF21 (H-105) SC-292879 1:2500 Santa Cruz Biotechnology PPARα (H-2) SC-398394 1:2500 Santa Cruz Biotechnology Sirt4 3224 1:2500 Bioworld Technology LaminB1 (L75) BS3547 1:2500 Bioworld Technology GAPDH (A531) AP0066 1:2500 Bioworld Technology Sirt5 (aa30-46) LS-B2060 1:2500 LifeSpan Biosciences Sirt7 ( aa317-366) LS-B1566 1:2500 LifeSpan Biosciences FoxO1 (Acetyl-Lys294) LS-C800723 1:2500 LifeSpan Biosciences Sirt2 (EPR20411-105) ab211033 1:2500 Abcam Sirt3 ab189860 1:2500 Abcam GRP78 BiP ab21685 1:2500 Abcam NCOR2/SMRT ab24551 1:2500 Abcam CPT1A (8F6AE9) ab128568 1:2500 Abcam T-OXPHOS ab110413 1:2500 Abcam THRβ ab53170 1:2500 Abcam p-IRE1α ab48187 1:2500 Abcam MDM2 (2A10) ab16895 1:2500 Abcam LXRα 14351-1-AP 1:2500 Proteintech Proximity Ligation Assay NCoR1 5948 1:100 Cell Signaling Technology p300 (D8Z4E) 86377 1:100 Cell Signaling Technology PPARα (H-2) SC-398394 1:100 Santa Cruz Biotechnology Immunofluorescence NCoR1 5948 1:100 Cell Signaling Technology PPARα (H-2) SC-398394 1:100 Santa Cruz Biotechnology Supplementary Table 4. ..

    Immunohistochemical staining:

    Article Title: p21-activated kinase 4 suppresses fatty acid β-oxidation and ketogenesis by phosphorylating NCoR1
    Article Snippet: .. Antibodies used for Western blotting, immunofluorescence and immunohistochemical analyses Antibody Catalogue No. Dilution Fold Company Western Blot PAK4 (G222) 62690 1:2500 Cell Signaling Technology p-PAK4 (S474) 3241 1:2500 Cell Signaling Technology HSP90 (C45G5) 4877 1:2500 Cell Signaling Technology Ubiquitin (E4I2J) 43124 1:2500 Cell Signaling Technology HMGCS2 (D3U1A) 20940 1:2500 Cell Signaling Technology Phospho-PKA Substrate (100G7E) 9624 1:2500 Cell Signaling Technology Phospho-(Ser/Thr) 9631 1:2500 Cell Signaling Technology Sirt1 2310 1:2500 Cell Signaling Technology Sirt6 (D8D12) 12486 1:2500 Cell Signaling Technology Phospho-Akt (Ser473) (D9E) 4060 1:2500 Cell Signaling Technology Akt 9272 1:2500 Cell Signaling Technology Acetylated-Lysine 9441 1:2500 Cell Signaling Technology CHOP (L63F7) 2895 1:2500 Cell Signaling Technology Phospho-S6 Ribosomal Protein (Ser240/244) 2215 1:2500 Cell Signaling Technology S6 Ribosomal Protein (5G10) 2217 1:2500 Cell Signaling Technology NCoR1 5948 1:2500 Cell Signaling Technology NEDD4 2740 1:2500 Cell Signaling Technology ATF-6 (D4Z8V) 65880 1:2500 Cell Signaling Technology Phospho-PERK (Thr980) (16F8) 3179 1:2500 Cell Signaling Technology PAK4 (B-3) SC-390507 1:2500 Santa Cruz Biotechnology FGF21 (H-105) SC-292879 1:2500 Santa Cruz Biotechnology PPARα (H-2) SC-398394 1:2500 Santa Cruz Biotechnology Sirt4 3224 1:2500 Bioworld Technology LaminB1 (L75) BS3547 1:2500 Bioworld Technology GAPDH (A531) AP0066 1:2500 Bioworld Technology Sirt5 (aa30-46) LS-B2060 1:2500 LifeSpan Biosciences Sirt7 ( aa317-366) LS-B1566 1:2500 LifeSpan Biosciences FoxO1 (Acetyl-Lys294) LS-C800723 1:2500 LifeSpan Biosciences Sirt2 (EPR20411-105) ab211033 1:2500 Abcam Sirt3 ab189860 1:2500 Abcam GRP78 BiP ab21685 1:2500 Abcam NCOR2/SMRT ab24551 1:2500 Abcam CPT1A (8F6AE9) ab128568 1:2500 Abcam T-OXPHOS ab110413 1:2500 Abcam THRβ ab53170 1:2500 Abcam p-IRE1α ab48187 1:2500 Abcam MDM2 (2A10) ab16895 1:2500 Abcam LXRα 14351-1-AP 1:2500 Proteintech Proximity Ligation Assay NCoR1 5948 1:100 Cell Signaling Technology p300 (D8Z4E) 86377 1:100 Cell Signaling Technology PPARα (H-2) SC-398394 1:100 Santa Cruz Biotechnology Immunofluorescence NCoR1 5948 1:100 Cell Signaling Technology PPARα (H-2) SC-398394 1:100 Santa Cruz Biotechnology Supplementary Table 4. ..

    Proximity Ligation Assay:

    Article Title: p21-activated kinase 4 suppresses fatty acid β-oxidation and ketogenesis by phosphorylating NCoR1
    Article Snippet: .. Antibodies used for Western blotting, immunofluorescence and immunohistochemical analyses Antibody Catalogue No. Dilution Fold Company Western Blot PAK4 (G222) 62690 1:2500 Cell Signaling Technology p-PAK4 (S474) 3241 1:2500 Cell Signaling Technology HSP90 (C45G5) 4877 1:2500 Cell Signaling Technology Ubiquitin (E4I2J) 43124 1:2500 Cell Signaling Technology HMGCS2 (D3U1A) 20940 1:2500 Cell Signaling Technology Phospho-PKA Substrate (100G7E) 9624 1:2500 Cell Signaling Technology Phospho-(Ser/Thr) 9631 1:2500 Cell Signaling Technology Sirt1 2310 1:2500 Cell Signaling Technology Sirt6 (D8D12) 12486 1:2500 Cell Signaling Technology Phospho-Akt (Ser473) (D9E) 4060 1:2500 Cell Signaling Technology Akt 9272 1:2500 Cell Signaling Technology Acetylated-Lysine 9441 1:2500 Cell Signaling Technology CHOP (L63F7) 2895 1:2500 Cell Signaling Technology Phospho-S6 Ribosomal Protein (Ser240/244) 2215 1:2500 Cell Signaling Technology S6 Ribosomal Protein (5G10) 2217 1:2500 Cell Signaling Technology NCoR1 5948 1:2500 Cell Signaling Technology NEDD4 2740 1:2500 Cell Signaling Technology ATF-6 (D4Z8V) 65880 1:2500 Cell Signaling Technology Phospho-PERK (Thr980) (16F8) 3179 1:2500 Cell Signaling Technology PAK4 (B-3) SC-390507 1:2500 Santa Cruz Biotechnology FGF21 (H-105) SC-292879 1:2500 Santa Cruz Biotechnology PPARα (H-2) SC-398394 1:2500 Santa Cruz Biotechnology Sirt4 3224 1:2500 Bioworld Technology LaminB1 (L75) BS3547 1:2500 Bioworld Technology GAPDH (A531) AP0066 1:2500 Bioworld Technology Sirt5 (aa30-46) LS-B2060 1:2500 LifeSpan Biosciences Sirt7 ( aa317-366) LS-B1566 1:2500 LifeSpan Biosciences FoxO1 (Acetyl-Lys294) LS-C800723 1:2500 LifeSpan Biosciences Sirt2 (EPR20411-105) ab211033 1:2500 Abcam Sirt3 ab189860 1:2500 Abcam GRP78 BiP ab21685 1:2500 Abcam NCOR2/SMRT ab24551 1:2500 Abcam CPT1A (8F6AE9) ab128568 1:2500 Abcam T-OXPHOS ab110413 1:2500 Abcam THRβ ab53170 1:2500 Abcam p-IRE1α ab48187 1:2500 Abcam MDM2 (2A10) ab16895 1:2500 Abcam LXRα 14351-1-AP 1:2500 Proteintech Proximity Ligation Assay NCoR1 5948 1:100 Cell Signaling Technology p300 (D8Z4E) 86377 1:100 Cell Signaling Technology PPARα (H-2) SC-398394 1:100 Santa Cruz Biotechnology Immunofluorescence NCoR1 5948 1:100 Cell Signaling Technology PPARα (H-2) SC-398394 1:100 Santa Cruz Biotechnology Supplementary Table 4. ..



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    Mettl18 deficiency induces unfolded protein response (UPR) activation and inflammatory cytokine expression. (A–D) Quantification of UPR pathway activation. Western blot analysis of whole pancreatic lysates from WT and KO mice using (A) anti-PERK (phospho-Thr980) and anti-PERK antibodies to assess PERK phosphorylation; (B) anti-EIF2 (phospho-Ser51) and anti-EIF2 antibodies to assess EIF2 phosphorylation; (C) anti-ATF4 antibody; and (D) anti-IRE1 (phospho-Ser724) and anti-IRE1 antibodies. Band intensities were quantified with ImageJ. 16-week-old; n = 6; mean ± SEM. Student's t -test: p∗∗<0.01, p∗<0.05. See also . (E) qPCR quantification of Xbp1s mRNA levels. Ct values were normalized to those obtained with primers detecting both Xbp1s and unspliced Xbp1 ( Xbp1u ) as described in . 16-week-old; n = 7; mean ± SEM. Student's t -test: n.s. > 0.1. See also . (F and G) qPCR quantification of mRNA expression of inflammatory cytokines, Il-1 β (E) and Il-6 (F). 16-week-old; n = 7; mean ± SEM. Student's t -test: p∗<0.05. (H) IL-1β protein levels were assessed by Western blotting of whole pancreatic lysates from WT and KO mice using an anti-IL-1β antibody. Quantification and statistical analyses were performed as in (A–D). (I) Schematic representation of UPR activation in WT and Mettl18 KO pancreas.

    Journal: Molecular Metabolism

    Article Title: METTL18 ensures pancreatic function by maintaining proper translation and proteostasis

    doi: 10.1016/j.molmet.2026.102337

    Figure Lengend Snippet: Mettl18 deficiency induces unfolded protein response (UPR) activation and inflammatory cytokine expression. (A–D) Quantification of UPR pathway activation. Western blot analysis of whole pancreatic lysates from WT and KO mice using (A) anti-PERK (phospho-Thr980) and anti-PERK antibodies to assess PERK phosphorylation; (B) anti-EIF2 (phospho-Ser51) and anti-EIF2 antibodies to assess EIF2 phosphorylation; (C) anti-ATF4 antibody; and (D) anti-IRE1 (phospho-Ser724) and anti-IRE1 antibodies. Band intensities were quantified with ImageJ. 16-week-old; n = 6; mean ± SEM. Student's t -test: p∗∗<0.01, p∗<0.05. See also . (E) qPCR quantification of Xbp1s mRNA levels. Ct values were normalized to those obtained with primers detecting both Xbp1s and unspliced Xbp1 ( Xbp1u ) as described in . 16-week-old; n = 7; mean ± SEM. Student's t -test: n.s. > 0.1. See also . (F and G) qPCR quantification of mRNA expression of inflammatory cytokines, Il-1 β (E) and Il-6 (F). 16-week-old; n = 7; mean ± SEM. Student's t -test: p∗<0.05. (H) IL-1β protein levels were assessed by Western blotting of whole pancreatic lysates from WT and KO mice using an anti-IL-1β antibody. Quantification and statistical analyses were performed as in (A–D). (I) Schematic representation of UPR activation in WT and Mettl18 KO pancreas.

    Article Snippet: Other antibodies used were as follows: anti-β-actin (clone 6D1, cat#M177-3; MBL); anti-phospho-PERK (Thr980) (clone G.305.4, cat#MA5-15033; Invitrogen); anti-RPL3 (cat#66130-1-Ig), anti-Reg1 (cat#15850-1-AP), and anti-PERK/EIF2AK3 (cat#24390-1-AP) (Proteintech Group); anti-IRE1 (cat#3294), anti-ATF4 (cat#11815), and anti-eIF5A (cat#20765) (Cell Signaling Technology); anti-phospho-IRE1 (cat#ab48187), anti-phospho-EIF2A (cat#ab32157), and anti-EIF2A (cat#ab5369) (Abcam); anti-IL-1β (cat#AF-401-NA; R&D Systems).

    Techniques: Activation Assay, Expressing, Western Blot, Phospho-proteomics